Proceedings of the National Academy of Sciences in the United States of America
National Academy of Sciences
According to the cisternal maturation hypothesis, endoplasmic reticulum (ER)-derived membranes nucleate new Golgi cisternae. The yeast Saccharomyces cerevisiae offers a unique opportunity to test this idea because small buds contain both ER and Golgi structures early in the cell cycle. We previously predicted that mutants defective in ER inheritance also would show defects in Golgi inheritance. Surprisingly, studies of S. cerevisiae have not revealed the expected link between ER and Golgi inheritance. Here, we revisit this issue by generating mutant strains in which many of the small buds are devoid of detectable ER. These strains also show defects in the inheritance of both early and late Golgi cisternae. Strikingly, virtually all of the buds that lack ER also lack early Golgi cisternae. Our results fit with the idea that membranes exported from the ER coalesce with vesicles derived from existing Golgi
compartments to generate new Golgi cisternae. This basic mech- anism of Golgi inheritance may be conserved from yeast to verte- brate cells.
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Catherine, Reinke; Patrycja, Kozik; Benjamin, Glick S.; and Gottfried, Schatz, "Golgi inheritance in small buds of Saccharomyces cerevisiae is linked to endoplasmic reticulum inheritance" (2004). Faculty Work. 9.