Proceedings of the National Academy of Sciences in the United States of America
National Academy of Sciences
According to the cisternal maturation hypothesis, endoplasmic reticulum (ER)-derived membranes nucleate new Golgi cisternae. The yeast Saccharomyces cerevisiae offers a unique opportunity to test this idea because small buds contain both ER and Golgi structures early in the cell cycle. We previously predicted that mutants defective in ER inheritance also would show defects in Golgi inheritance. Surprisingly, studies of S. cerevisiae have not revealed the expected link between ER and Golgi inheritance. Here, we revisit this issue by generating mutant strains in which many of the small buds are devoid of detectable ER. These strains also show defects in the inheritance of both early and late Golgi cisternae. Strikingly, virtually all of the buds that lack ER also lack early Golgi cisternae. Our results fit with the idea that membranes exported from the ER coalesce with vesicles derived from existing Golgi
compartments to generate new Golgi cisternae. This basic mech- anism of Golgi inheritance may be conserved from yeast to verte- brate cells.
Carleton College does not own the copyright to this work and the work is available through the Carleton College Library following the original publisher's policies regarding self-archiving. For more information on the copyright status of this work, refer to the current copyright holder.
Publisher PDF Archiving
Paid OA Option
Catherine, Reinke, Kozik Patrycja, Glick S. Benjamin, and Schatz Gottfried. 2004. "Golgi Inheritance in Small Buds of Saccharomyces Cerevisiae Is Linked to Endoplasmic Reticulum Inheritance." Proceedings of the National Academy of Sciences in the United States of America 101, (52): 18018-18023. Available at: https://doi.org/10.1073/pnas.0408256102. Accessed via Faculty Work. Biology. Carleton Digital Commons. https://digitalcommons.carleton.edu/biol_faculty/9
The definitive version is available at https://doi.org/10.1073/pnas.0408256102