Journal Title

Proceedings of the National Academy of Sciences in the United States of America

Carleton Author

Reinke, Catherine

Department

Biology

Publication Date

2004

Volume No.

101

First Page

18018

Last Page

18023

Publisher

National Academy of Sciences

File Name

003_Reinke-Catherine_GolgiInheritanceInSmallBudsOfSaccharomyces.pdf

Abstract

According to the cisternal maturation hypothesis, endoplasmic reticulum (ER)-derived membranes nucleate new Golgi cisternae. The yeast Saccharomyces cerevisiae offers a unique opportunity to test this idea because small buds contain both ER and Golgi structures early in the cell cycle. We previously predicted that mutants defective in ER inheritance also would show defects in Golgi inheritance. Surprisingly, studies of S. cerevisiae have not revealed the expected link between ER and Golgi inheritance. Here, we revisit this issue by generating mutant strains in which many of the small buds are devoid of detectable ER. These strains also show defects in the inheritance of both early and late Golgi cisternae. Strikingly, virtually all of the buds that lack ER also lack early Golgi cisternae. Our results fit with the idea that membranes exported from the ER coalesce with vesicles derived from existing Golgi

compartments to generate new Golgi cisternae. This basic mech- anism of Golgi inheritance may be conserved from yeast to verte- brate cells.

Rights Management

Yes

RoMEO Color

RoMEO_Color_Green

Preprint Archiving

Yes

Postprint Archiving

Yes

Publisher PDF Archiving

No

Contributing Organization

Carleton College

Type

Article

Format

application/pdf

Language

English

DOI

doi:10.1073/pnas.0408256102

Included in

Biology Commons

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