Type

Article

Abstract

According to the cisternal maturation hypothesis, endoplasmic reticulum (ER)-derived membranes nucleate new Golgi cisternae. The yeast Saccharomyces cerevisiae offers a unique opportunity to test this idea because small buds contain both ER and Golgi structures early in the cell cycle. We previously predicted that mutants defective in ER inheritance also would show defects in Golgi inheritance. Surprisingly, studies of S. cerevisiae have not revealed the expected link between ER and Golgi inheritance. Here, we revisit this issue by generating mutant strains in which many of the small buds are devoid of detectable ER. These strains also show defects in the inheritance of both early and late Golgi cisternae. Strikingly, virtually all of the buds that lack ER also lack early Golgi cisternae. Our results fit with the idea that membranes exported from the ER coalesce with vesicles derived from existing Golgi

compartments to generate new Golgi cisternae. This basic mech- anism of Golgi inheritance may be conserved from yeast to verte- brate cells.

Language

English

Department(s)

Biology

Journal or Book Title

Proceedings of the National Academy of Sciences in the United States of America

Publication Year

2004

DOI

10.1073/pnas.0408256102

Publisher

National Academy of Sciences

Rights Management

Carleton College does not own the copyright to this work and the work is available through the Carleton College Library following the original publisher policies regarding self-archiving. For more information on the copyright status of this work, refer to the current copyright holder.

RoMEO Color

Green

Preprint Archiving

Yes

Postprint Archiving

Yes

Publisher PDF Archiving

No

Contributing Organization

Carleton College

Format

application/pdf

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