Type
Article
Keywords
Biochemistry, molecular biology, mutagenesis, undergraduate
Abstract
Site-directed mutagenesis and other molecular biology techniques, including plasmid manipulation and restriction analysis, are commonly used tools in the biochemistry research laboratory. In redesigning our biochemistry lab curricula, we sought to integrate these techniques into a term-long, project-based course. In the module presented here, students use structural data to design a site-directed mutant and make the mutation using the Ku¨ nkel method. A second, silent mutant, that creates or removes a restriction site, is simultaneously introduced. Restriction digestion and agarose gel electrophoresis are used to assess the success of mutagenesis. Placing these procedures in the context of continuous, studentdriven project serves to create a ‘‘research style’’ laboratory environment.
Language
English
Department(s)
Chemistry
Journal or Book Title
Biochemistry and Molecular Biology Education
Publication Year
2008
DOI
10.1002/bmb.20170
Publisher
John Wiley & Sons
Rights Management
Carleton College does not own the copyright to this work and the work is available through the Carleton College Library following the original publisher policies regarding self-archiving. For more information on the copyright status of this work, refer to the current copyright holder.
RoMEO Color
Green
Preprint Archiving
Yes
Postprint Archiving
Yes
Publisher PDF Archiving
No
Paid OA Option
Yes
Contributing Organization
Carleton College
Format
application/pdf
Recommended Citation
Muth, Gregory W., and Joseph W. Chihade., "A Streamlined Molecular Biology Module for Undergraduate Biochemistry Labs". Biochemistry and Molecular Biology Education. 2008, 363: 209-216. Available at: https://doi.org/10.1002/bmb.20170. Accessed via Faculty Work. Chemistry. Carleton Digital Commons. https://digitalcommons.carleton.edu/chem_faculty/3
The definitive version is available at https://doi.org/10.1002/bmb.20170
