Carleton Author

Chihade, Joseph W.

Department

Chemistry

Journal Title

Biochemistry and Molecular Biology Education

Publication Date

2008

Volume No.

36

Issue No.

3

First Page

209

Last Page

216

Publisher

John Wiley & Sons

File Name

080_Chihade-Joseph_AStreamlinedMolecularBiologyModuleForUndergraduateBiochemistryLabs.pdf

Keywords

Biochemistry, molecular biology, mutagenesis, undergraduate

Abstract

Site-directed mutagenesis and other molecular biology techniques, including plasmid manipulation and restriction analysis, are commonly used tools in the biochemistry research laboratory. In redesigning our biochemistry lab curricula, we sought to integrate these techniques into a term-long, project-based course. In the module presented here, students use structural data to design a site-directed mutant and make the mutation using the Ku¨ nkel method. A second, silent mutant, that creates or removes a restriction site, is simultaneously introduced. Restriction digestion and agarose gel electrophoresis are used to assess the success of mutagenesis. Placing these procedures in the context of continuous, studentdriven project serves to create a ‘‘research style’’ laboratory environment.

Rights Management

Carleton College does not own the copyright to this work and the work is available through the Carleton College Library following the original publisher's policies regarding self-archiving. For more information on the copyright status of this work, refer to the current copyright holder.

RoMEO Color

Green

Preprint Archiving

Yes

Postprint Archiving

Yes

Publisher PDF Archiving

No

Contributing Organization

Carleton College

Type

Article

Format

application/pdf

Language

English

DOI

10.1002/bmb.20170

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Chemistry Commons

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